Method for preparing immunoglobulin fragments
AssigneeUniSyn Technologies, Inc.
InventorsThat T. Ngo, Harish P. M. Kumar
Life of this patent
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Abstract
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising treating a solution of immunoglobulins with a soluble protease so as to achieve substantially complete fragmentation of the immunoglobulins, followed by complexing the protease with a complexing agent (for example, antibody to the protease) to form a protease complex and separating the protease complex from the immunoglobulin fragments. Optionally, the protease (e.g., pepsin or papain) is inactivated prior to formation of the protease complex. In contrast to methods which use immobilized forms of the enzymes, the rate of cleavage and the final yields of F.sub.(ab) and F.sub.(ab')2 fragments are increased substantially.
Claims11
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising:
- treating a solution of said immunoglobulins with a protease soluble in said solution so as to achieve substantially complete fragmentation of said immunoglobulins;
- complexing said protease with a complexing agent to form a protease complex;
- separating said protease complex from said immunoglobulin fragments;
- and
- inactivating said protease prior to said complexing to ensure that said complexing agent is not digested by said protease;
- wherein said protease is papain and said inactivating is effected by addition of a papain inhibitor.
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising:
- treating a solution of said immunoglobulins with a protease soluble in said solution so as to achieve substantially complete fragmentation of said immunoglobulins;
- complexing said protease with a complexing agent to form a protease complex;
- separating said protease complex from said immunoglobulin fragments;
- and
- inactivating said protease prior to said complexing to ensure that said complexing agent is not digested by said protease;
- wherein said protease is papain and said inactivating is effected by addition of antipain or leupeptin.
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising:
- treating a solution of said immunoglobulins with a protease soluble in said solution so as to achieve substantially complete fragmentation of said immunoglobulins;
- complexing said protease with a complexing agent to form a protease complex;
- and
- separating said protease complex from said immunoglobulin fragments;
- wherein said protease is biotinylated papain and said complexing agent is avidin.
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulins, comprising:
- treating a solution of said immunoglobulins with a protease soluble in said solution so as to achieve substantially complete fragmentation of said immunoglobulins;
- complexing said protease with a complexing agent to form a protease complex;
- and
- separating said protease complex from said immunoglobulin fragments;
- wherein said protease is biotinylated papain and said complexing agent is avidin and said complexing and said separating are carried out using an avidin-agarose column.
A method for preparation of immunoglobulin fragments by specific fragmentation of immunoglobulin, comprising:
- treating a solution of said immunoglobulins with a protease soluble in said solution so as to achieve substantially complete fragmentation of said immunoglobulins;
- complexing said inactivated protease with a complexing agent which forms a protease complex but does not form a complex with fragments of said immunoglobulins;
- said protease having been inactivated prior to said complexing to insure that said complexing agent is not digested by said protease;
- and
- separating said protease complex from said immunoglobulin fragments.
Dependencies are inferred from the claim text.