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US 5,328,825 AGranted
Nucleic acid probe, test kit and diagnostic and purification methods
AssigneeEastman Kodak Company
InventorsHarold C. Warren, III, John B. Findlay, Marlene M. King
Life of this patent
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Abstract
An oligonucleotide is linked to a particle through a protein or carbohydrate to form a water-insoluble nucleic acid probe. The protein or carbohydrate has a pI of about 6 or less, and has been chemically modified with an acylating, alkylating or sulfonylating agent. The particle surface is substantially free of other proteins or carbohydrates. The probe is useful in various diagnostic and purification methods wherein hybridization of the oligonucleotide with a target nucleic acid is possible. In one instance, the probe can be used to capture a DNA strand which has been amplified using polymerase chain reaction techniques.
Claims19
A water-insoluble nucleic acid probe comprising an oligonucleotide which is attached to a water-insoluble particle through a linking group which is a chemically modified protein or a chemically modified carbohydrate having a pI of about 6 or less,
- said chemically modified protein having been derived from a protein having pendant amino groups which have been chemically modified with an acrylating, alkylating or sulfonylating agent to provide carboxylic acid groups for covalent reaction with said oligonucleotide, and said chemically modified carbohydrate having been modified to have pendant carboxylic acid groups, and said chemically modified protein or chemically modified carbohydrate being covalently attached to said particle surface which is substantially free of other proteins or carbohydrates.
A method for capturing and isolating a target nucleic acid comprising:
- A. contacting a test sample suspected of containing a target nucleic acid with a water-insoluble nucleic acid probe comprising an oligonucleotide which is attached to a water-insoluble particle through a linking group which is a chemically modified protein or a chemically modified carbohydrate having a pI of about 6 or less,
- said chemically modified protein having been derived from a protein having a pendant amino groups which have been chemically modified with an acylating, alkylating or sulfonylating agent to provide carboxylic acid groups for covalent reaction with said oligonucleotide, and said chemically modified carbohydrate having been modified to have pendant carboxylic acid groups, and said chemically modified protein or chemically modified carbohydrate being covalently attached to said particle surface which is substantially free of other of other proteins or carbohydrates,
- said oligonucleotide being complementary to said target nucleic acid,
- said contacting being carried out under hybridization conditions so that hybridization of said target nucleic acid and said oligonucleotide is achieved to form a water-insoluble hybridization product, and
- B. isolating said hybridized product from the rest of said test sample.
2 more elements
A method for the detection of a target nucleic acid comprising:
- A. contacting a test sample suspected of containing a target nucleic acid with a water-insoluble nucleic acid probe comprising an oligonucleotide which is attached to a water-insoluble particle through a linking group which is a chemically modified protein or a chemically modified carbohydrate having a pI of about 6 or less,
- said chemically modified protein having been derived from a protein having pendant amino groups which have been chemically modified with an acylating, alkylating or sulfonylating agent to provide carboxylic acid groups for covalent reaction with said oligonucleotide, and said chemically modified carbohydrate having been modified to have pendant carboxylic acid groups, and said chemically modified protein or chemically modified carbohydrate being covalently attached to said particle surface which is substantially free of other proteins or carbohydrates,
- said oligonucleotide being complementary and specific to said target nucleic acid,
- said contacting being carried out under hybridization conditions so that hybridization of said target nucleic acid and said oligonucleotide is achieved to form a water-insoluble hybridization product, and
- B. detecting said hybridized product as an indication of the presence of said target nucleic acid in said test sample.
2 more elements
A method for the detection of a target nucleic acid, said method comprising:
- A. amplifying a target nucleic acid in a test specimen in the presence of complementary primers, deoxyribonucleotide triphosphates and a polymerization agent,
- B. contacting said amplified target nucleic acid with a water-insoluble nucleic acid probe comprising an oligonucleotide which is attached to a water-insoluble particle through a linking group which is a chemically modified protein or a chemically modified carbohydrate having a pI of about 6 or less,
- said chemically modified protein having been derived form a protein having pendant amino groups which have been chemically modified with an acylating, alkylating or sulfonylating agent to provide carboxylic acid groups for covalent reaction with said oligonucleotide, and said chemically modified carbohydrate having been modified to have pendant carboxylic acid groups, and said chemically modified protein or chemically modified carbohydrate being covalently attached to said particle surface which is free of other proteins or carbohydrates,
- to form an immobilized hybridized product of target nucleic acid and said water-insoluble probe,
- C. separating said immobilized product from nonimmobilized materials, and
- D. detecting said immobilized product as an indication of the amount of target nucleic acid in said specimen.
3 more elements
A diagnostic test kit comprising:
- (a) a water-insoluble nucleic acid probe comprising an oligonucleotide which is attached to a water-insoluble particle through a linking group which is a chemically modified protein or a chemically modified carbohydrate having a pI of about 6 or less,
- said chemically modified protein having been derived from a protein having pendant amino groups which have been chemically modified with an acrylating, alkylating or sulfonylating agent to provide carboxylic acid groups for covalent reaction with said oligonucleotide, and said chemically modified carbohydrate having been modified to have pendant carboxylic acid groups, and said chemically modified protein or chemically modified carbohydrate being covalently attached to said particle surface which is substantially free of other proteins or carbohydrates, and
- (b) a water-soluble primer or probe which is complementary and specific to a target nucleic acid, said target nucleic acid also being complementary and specific to said oligonucleotide of component (a).
Dependencies are inferred from the claim text.