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US 5,328,821 AGranted
Cold and cryo-preservation methods for human tissue slices
OwnerRobyn Fisher, Klaus Brendelinventors
Life of this patent
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Abstract
Cold-and cryo-preservation solutions for tissue slices include a limited amount of glucose. The solutions provide extended cold storage of the slices without a loss of viability. The solutions also allow cryopreservation of the slices, so that the slices may be stored until needed and then thawed, cultured, packaged and distributed.
Claims7
A method for the cold preservation of organ tissue comprising storing said tissue in an aqueous preservation solution comprising:
- 1-5 mM glucose;
- 30-120 mM gluconate;
- 30-120 mM saccharate;
- 25-30 mM potassium phosphate(monobasic);
- 1-3 mM sodium succinate;
- 3-6 mM magnesium sulfate;
- 1-3 mM magnesium chloride;
- 1-3 mM potassium bicarbonate;
- 2-4 mM fructose;
- 3-6 mM glutathione;
- 2-10 mM ascorbic acid;
- 3-5 mM adenosine and micromolar amounts of deferoxamine mesylate and gentamicin or penicillin.
9 more elements
The method of 1 wherein the solution further comprises a cryoprotectant and the method further comprises immersing the preserved tissue into a crygenic fluid.
Dependencies are inferred from the claim text.