ABO genotyping
AssigneeThe Biomembrane Institute
InventorsFumi-ichiro Yamamoto, Thayer White, Sen-itiroh Hakomori, Henrik Clausen
Life of this patent
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Abstract
The genes defining the ABO histo-blood groups are disclosed. Methods for identification of histo-blood group ABO status are provided. The methods include the use of DNA probes or size separation of DNA fragments unique to a blood group status. The present invention also discloses DNA constructs, recombinant methods for providing histo-blood glycosyltransferases, methods for tumor suppression, purified histo-blood group glycosyltransferases, and antibodies produced therefrom which bind to protein epitopes.
Claims30
An isolated DNA molecule encoding a human histo-blood group A glycosyltransferase.
An isolated DNA molecule encoding a human histo-blood group A glycosyltransferase wherein said glycosyltransferase consists of the amino acid sequence of FIG. 3 from alanine, amino acid 54, to proline, amino acid 353.
An isolated DNA molecule encoding a human histo-blood group A glycosyltransferase wherein said DNA molecule consists of a sequence of nucleotides as shown in FIG. 3 from nucleotide 160 to nucleotide 1059.
An isolated DNA molecule encoding a human histo-blood group A glycosyltransferase wherein said glycosyltransferase consists of the amino acid sequence of FIG. 3 from methionine, amino acid 1, to proline, amino acid 353.
An isolated DNA molecule encoding a human histo-blood group A glycosyltransferase wherein said DNA molecule consists of a sequence of nucleotides as shown in FIG. 3 from nucleotide 1 to nucleotide 1059.
An isolated DNA molecule encoding a human histo-blood group B glycosyltransferase.
An isolated DNA molecule consisting of the nucleotide sequence of the human histo-blood group O glycosyltransferase gene.
A DNA construct comprising a DNA sequence encoding a protein having the activity of a human histo-blood group A glycosyltransferase.
A recombinant plasmid comprising a DNA sequence encoding human histo-blood group A glycosyltransferase.
A recombinant plasmid which expresses histo-blood group A glycosyltransferase, said plasmid comprising a promoter followed downstream by a DNA sequence encoding human histo-blood group A glycosyltransferase, said DNA sequence being followed downstream by a polyadenylation signal.
Cells stably transfected with a recombinant plasmid comprising a DNA sequence a sequence encoding human histo-blood group A glycosyltransferase, said cells producing said glycosyltransferase in recoverable amounts.
A DNA construct comprising a DNA sequence encoding a protein having the activity of a human histo-blood group B glycosyltransferase.
A recombinant plasmid comprising a DNA sequence encoding human histo-blood group B glycosyltransferase.
A recombinant plasmid which expresses histo-blood group B glycosyltransferase, said plasmid comprising a promoter followed downstream by a DNA sequence encoding human histo-blood group B glycosyltransferase, said DNA sequence being followed downstream by a polyadenylation signal.
Cells stably transfected with a recombinant plasmid comprising a DNA sequence encoding human histo-blood group B glycosyltransferase, said cells producing said glycosyltransferase in recoverable amounts.
A DNA construct comprising a DNA sequence encoding a protein having the activity of a human histo-blood group A glycosyltransferase and the activity of a human histo-blood group B glycosyltransferase.
Dependencies are inferred from the claim text.