Serum-free medium for culturing animal cells
AssigneeKurashiki Boseki Kabushiki Kaisha
InventorsHisashi Torishima, Hirokuni Arakawa, Ryohei Yamamoto, Toyokazu Nishino, Chikaaki Sakai
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Abstract
The present invention provides a serum-free medium for culturing animal cells characterized in that it comprises 8.0-14.0 mg/l of methionine. Although a wide variety of animal cells can effectively be cultured by means of the serum-free medium according to the present invention, said serum-free medium produces an excellent growth effect for culturing animal epithelial cells in particular.
Claims18
A serum-free medium for culturing animal epithelial cells comprising 9.0-14.0 mg/l of methionine, 5.0-11.5 mg/l of threonine, 7.0-11.0 mg/l of tyrosine, 9.0-14.5 mg/l of phenylalanine, 400.0-550.0 mg/l of glutamine, 7.0-9.5 mg/l of aspartic acid, 19.0-28.0 mg/l of lysine HCl, 33.0-40.0 mg/l of serine, and up to 0.1 mM of Ca in the form of calcium chloride;
- and one or more conventional ingredients selected from the group consisting of glucose, vitamins, minerals, growth factors, HEPES, and amino acids other than the foregoing listed amino acids.
A serum free medium for culturing animal epithelial cells which comprises 9.0-14.0 mg/l of methionine, 5.0-11.5 mg/l of threonine, 7.0-11.0 mg/l of tyrosine, 9.0-14.5 mg/l of phenylalanine, 400.0-550.0 mg/l of glutamine, 7.0-9.5 mg/l of aspartic acid, 19.0-28.0 mg/l of lysine HCl, 33.0-40.0 mg/l of serine, 0.1 mM or less of calcium chloride;
- alanine, arginine HCl, asparagine H.sub.2 O, cysteine HCl H.sub.20, glutamic acid, glycine, histidine HCl H.sub.2 O, isoleucine, leucine, proline, tryptophan, valine, biotin, folic acid, lipoic acid, nicotinamide, calcium pantothenate, pyridoxine HCl, riboflavin, thiamine HCl, vitamin B.sub.12, adenine, choline chloride, inositol, hypoxanthine, putrescine HCl, sodium pyruvate, thymidine, KCl, MgCl.sub.2 6H.sub.2 O, NaCl, NaH.sub.2 PO.sub.4 7H.sub.2 O, KH.sub.2 PO.sub.4, CuSO.sub.4 5H.sub.2 O, FeSO.sub.4 7H.sub.2 O, Na.sub.2 SeO.sub.4, MnSO.sub.4 5H.sub.2 O, Na.sub.2 SiO.sub.3 9H.sub.2 O, (NH.sub.4).sub.6 Mo.sub.7 O.sub.24 4H.sub.2 O, NH.sub.4 VO.sub.3, NiCl.sub.2 6H.sub.2 O, SnCl.sub.2 2H.sub.2 O, ZnSO.sub.4 7H.sub.2 O, NaHCO.sub.3 glucose, HEPES, phenol red (Na), insulin, hydrocortisone, epidermal growth factor, and bovine pituitary extract.
A method of culturing animal corneal epithelial cells comprising culturing said cells in a serum-free medium comprising 9.0-14.0 mg/l of methionine, 5.0-11.5 mg/l of threonine, 7.0-11.0 mg/l of tyrosine, 9.0-14.5 mg/l of phenylalanine, 400.0-550.0 mg/l of glutamine, 7.0-9.5 mg/l of aspartic acid, 19.0-28.0 mg/l of lysine HCl, 33.0-40.0 mg/l of serine, and up to 0.1 mM of Ca in the form of calcium chloride;
- and one or more conventional ingredients selected from the group consisting of glucose, vitamins, minerals, growth factors, HEPES, and amino acids other than the foregoing listed amino acids.
A method of culturing animal corneal epithelial cells which comprises culturing said cells in a serium-free medium comprising 9.0-14.0 mg/l of methionine, 5.0-11.5 mg/l of threonine, 7.0-11.0 mg/l of tyrosine, 9.0-14.5 mg/l of phenylalanine, 400.0-550.0 mg/l of glutamine, 7.0-9.5 mg/l of aspartic acid, 19.0-28.0 mg/l of lysine HCl, 33.0-40.0 mg/l of serine, 0.1 mM or less of calcium chloride;
- alanine, arginine HCl asparagine H.sub.2 O, cysteine HCl H.sub.2 O, glutamic acid, glycine, histidine HCl H.sub.2 O, isoleucine, leucine, proline, tryptophan, valine, biotin, folic acid, lipoic acid, nicotinamide, calcium pantothenate, pyridoxine HCl, riboflavin, thiamine HCl, vitamin B.sub.12, adenine, choline chloride, inositol, hypoxanthine, putrescine HCl, sodium pyruvate, thymidine, KCl, MgCl.sub.2 6H.sub.2 O, NaCl, NaH.sub.2 PO.sub.4 7H.sub.2 O, KH.sub.2 PO.sub.4, CuSO.sub.4 5H.sub.2 O, FeSO.sub.4 7H.sub.2 O, Na.sub.2 SeO.sub.4, MnSO.sub.4 5H.sub.2 O, Na.sub.2 SiO.sub.3 9H.sub.2 O, (NH.sub.4).sub.6 Mo.sub.7 O.sub.24 4H.sub.2 O, NH.sub.4 VO.sub.3, NiCl.sub.2 6H.sub.2 O, SnCl.sub.2 2H.sub.2 O, ZnSO.sub.4 7H.sub.2 O, NaHCO.sub.3, glucose, HEPES, phenol red (Na), insulin, hydrocortisone, epidermal growth factor, and bovine pituitary extract.
Dependencies are inferred from the claim text.