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US 5,326,698 AGranted
Method of purification of penicillin amidase using phenylbutylamine-eupergit
AssigneeRoehm GmbH Chemische Fabrik
InventorsVolker Kasche, Fridolin Loffler, Dieter Kramer, Gerd Janowski
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Abstract
An enzyme is purified by absorbing enzymes in an aqueous solution on a specific carrier material T-PbA to which is covalently bound phenyl butylamine ligand groups.
Claims10
A method for purification of penicillin amidase on substrates which contain aromatic groups, comprising:
- absorbing pencillin amidase in a buffered aqueous solution at a pH near neutral on a specific carrier material T-PbA by hydrophobic interaction, said carrier material prepared by covalently bonding phenylbutylamine to a carrier pre-stage T-Vs-A by reaction of the phenylbutylamine with glycidyl groups on the carrier, which glycidyl groups constitute the activated group on the carrier substrate;
- and
- eluting the absorbed enzyme from the carrier substrate with a buffered aqueous acid eluting solution.
Dependencies are inferred from the claim text.