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US 5,326,558 AGranted
Megakaryocytopoietic factor
AssigneeGenetics Institute, Inc.
InventorsKatherine Turner, Steven C. Clark, Thomas G. Gesner, Rodney M. Hewick
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Abstract
A novel human megakaryocytopoietic factor capable of stimulating the growth and development of colonies of megakaryocytes is provided, including procedures for its purification and use as a pharmaceutical agent.
Claims7
A human megakaryocyte colony stimulating factor protein, substantially free from association with other proteinaceous material, said protein being characterized by possessing
- (a) the ability to stimulate growth and development of colonies consisting of megakaryocyte cells;
- (b) an apparent molecular weight of about 28-38 kd as determined by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis under non-reducing conditions;
- (c) an apparent molecular weight of about 20-27 kd as determined by 12% sodium dodecyl sulfate polyacrylamide gel electrophoresis under reducing conditions;
- and
- (d) a partial amino acid sequence selected from the group consisting of:
- (i) Ser Arg Cys Phe Glu Ser Phe Glu Arg;
- (ii) Arg Val Cys Thr Ala Glu Leu Ser Cys Lys Gly;
- (iii) Lys Ala Pro Pro (X) Gly Ala Ser Gln Thr Ile Lys;
- and
- (iv) Lys Tyr Asp Lys Cys Cys Pro Asp Tyr Glu Ser Phe Cys Ala Glu Val His Asn Pro, wherein (X)=Ser or Thr.
7 more elements
Dependencies are inferred from the claim text.