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US 5,324,656 AGranted
Media for normal human muscle satellite cells
AssigneeUniversity of Colorado Foundation, Inc.
InventorsRichard G. Ham, Judith A. St. Clair, Zetan Nie
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AssigneeUniversity of Colorado Foundation, Inc.
InventorsRichard G. Ham, Judith A. St. Clair, Zetan Nie
Estimating the term…
An improved basal nutrient medium MCDB 120 for the clonal growth of human muscle satellite cells (HMSC) is described. In addition, serum-free supplements for the clonal growth of HMSC containing serum albumin, dexamethasone, fetuin fraction mitogen, epidermal growth factor and insulin are described. Combination of MCDB 120 and the serum-free supplement results in a serum-free medium for growth of HMSC, which can be transplanted to muscles of patients afflicted with muscle degenerative diseases such as muscular dystrophy. The serum-free supplement can also be used with other basal nutrient media such as MCDB 131M for growth of HMSC. Addition of 5% dialyzed fetal bovine serum to the serum-free media described results in semi-defined media which allows significantly improved growth of HMSC.
Human muscle satellite cell growth medium MCDB 120.
A method of growing human muscle satellite cell progeny comprising the step of growing a human muscle satellite cell in a culture comprising:
A serum-free supplement comprising dexamethasone, serum albumin, epidermal growth factor and a mitogen preparation selected from the group consisting of fraction 50 PEG 2X comprising fetuin-replacing activity and combined FPLC anion exchange fractions F14+15+16 comprising fetuinreplacing activity purified from said fraction 50 PEG 2X.
Dependencies are inferred from the claim text.