Fibrin coated polymer surfaces
AssigneesMcMaster University, ZymoGenetics, Inc.
InventorsFraser D. Rubens, Paul D. Bishop
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Abstract
Surfaces of polymeric materials are coated with a layer of thermally denatured fibrinogen. The fibrinogen may be treated with thrombin to produce fibrin, and additional fibrinogen and factor XIII added to produce a cross-linked fibrin coating. The resulting coated surface is stable, anti-thrombotic and resistant to platelet adhesion. Polymeric materials coated in this way are useful in constructing vascular grafts and prosthetic devices, and provide test systems useful in the study of thrombogenesis.
Claims10
In a method of coating a surface of a polymeric material with fibrin, the improvement comprising:
- incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56.degree. C. but less than 100.degree. C. to produce a fibrinogen-coated surface;
- treating the fibrinogen-coated surface with thrombin to produce fibrin monomers;
- and then exposing the thrombin-treated surface to a solution comprising factor XIII and additional fibrinogen, whereby the additional fibrinogen is converted to fibrin and cross-linked to the fibrin-coated surface.
A polymeric material prepared according to the method of incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56.degree. C. but less than 100.degree. C. to produce a fibrinogen-coated surface.
A polymeric material prepared according to the method of:
- incubating said polymeric material in a solution of fibrinogen at a temperature of at least 56.degree. C. but less than 100.degree. C. to produce a fibrinogen-coated surface;
- and then
- treating the fibrinogen-coated surface with thrombin to produce fibrinogen monomers.
Dependencies are inferred from the claim text.