Cephalosporin C acylase
AssigneeFujisawa Pharmaceutical Co., Ltd.
InventorsMorita Iwami, Ichiro Aramori, Masao Fukagawa, Takao Isogai, Hitoshi Kojo
Life of this patent
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Abstract
A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.
Claims7
An isolated and purified DNA consisting essentially of a nucleotide sequence which encodes a cephalosporin C acylase having the following characteristics:
- (a) enzymatically converts cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin C, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid,
- (b) contains (i) an .alpha.-subunit having a molecular weight of 26,000 daltons as determined by SDS-PAGE and (ii) a .beta.-subunit having a molecular weight of 58,000 daltons as determined by SDS-PAGE,
- (c) said .alpha.-subunit has an N-terminal amino acid sequence (SEQ ID NO:1) of the formula:
- ##STR3##
Dependencies are inferred from the claim text.