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US 5,320,725 AGranted
Electrode and method for the detection of hydrogen peroxide
AssigneeE. Heller & Company
InventorsBrian A. Gregg, Adam Heller
Life of this patent
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Abstract
An amperometric biosensor and method for the detection of hydrogen peroxide, NADH, or NADPH with high sensitivity includes an electrode having on its testing surface a three-dimensional redox polymer network in which peroxidase is immobilized.
Claims17
An electrode for the detection of hydrogen peroxide comprising:
- an electrode having a testing surface;
- and
- a transducing film substantially covering the testing surface of the electrode, the film comprising a cross-linked redox polymer network, the network comprising a redox compound having multiple redox centers and a peroxidase having redox centers wherein the redox centers of the peroxidase are electrically connected, in the absence of diffusional electron shuttles, to the electrode.
A method for analyzing the presence of hydrogen peroxide in a test sample comprising the steps of:
- contacting a test sample with an electrode having a test surface substantially covered with a transducing film comprising a cross-linked redox polymer network having multiple redox centers and peroxidase immobilized in the network;
- generating electrons at the electrode;
- transferring electrons from the electrode through the cross-linked polymer network to the peroxidase;
- electroreducing hydrogen peroxide at the peroxidase;
- generating an electrical current by electroreduction of hydrogen peroxide within the cross-linked redox polymer network;
- and
- measuring the generated current.
4 more elements
A method for analyzing the presence of NADPH or NADH in a test sample comprising the steps of:
- reacting NADPH or NADH with dissolved molecular oxygen in the presence of a quinoid in the test sample;
- contacting the reacted test solution with an electrode having a testing surface substantially covered with a transducing film comprising a cross-linked redox polymer network having multiple redox centers and peroxidase immobilized in the network;
- generating electrons at the electrode;
- transferring electrons from the electrode through the cross-linked polymer network to the peroxidase;
- electroreducing hydrogen peroxide at the immobilized peroxidase;
- generating an electrical current by the transfer of electrons and electroreduction of hydrogen peroxide;
- and
- quantifying the generated current.
5 more elements
Dependencies are inferred from the claim text.