Aspergillus niger mutants
AssigneeMerck & Co., Inc.
InventorsJennifer N. Kahn, Kevin M. Byrne, Louis Kaplan, Richard J. Monaghan
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Abstract
Disclosed are new Aspergillus niger mutants which can be used for identifying active FK-506/ rapamycin type, or cyclosporin A type inununosuppressants in a broth, natural product extract, or composition exhibiting inununosuppressant activity. Specifically disclosed are the new mutants, ATCC No. 74085 (MF 5659), which is sensitive to FK-506 and rapamycin but resistant to cyclosporin A; ATCC No. 74086 (MF 5659) which is resistant to FK-506, cyclosporin A and rapamycin; and ATCC No. 74087 (MF 5661) which is resistant to FK-506 and rapamycin, but sensitive to cyclosporin A.
Claims6
A biologically pure form of an Aspergillus niger mutant identified as ATCC No. 74085.
A biologically pure form of an Aspergillus niger mutant identified as ATCC No. 74086.
A biologically pure form of an Aspergillus niger mutant identified as ATCC No. 74087.
A process for testing a compound or fermentation broth for cyclosporin immunosuppressant-type activity comprising the steps of:
- (1) contacting said compound or broth with:
- (a) Aspergillus niger ATCC No. 74085 mutant, and (b) AspergillUS niger ATCC No. 6275;
- (2) observing the conidiation characteristics of said ATCC No. 74085 mutant, which are positive in the presence of cyclosporin immunosuppressive-type activity, and (3) observing the conidiation characteristics of said ATCC No. 6275 which are negative in the presence of cyclosporin.
A process for testing a compound or fermentation broth for FK-506/rapamycin immunosuppressant-type activity comprising the steps of:
- (1) contacting said compound or broth with (a) Aspergillus niger ATCC No. 74087 mutant and (b) Aspergillus niger ATCC No. 6275, and (2) observing the conidiation characteristics of said ATCC No. 74087 mutant, which are positive in the presence of FK-506/ rapamycin immunosuppressive-type activity, and (3) observing the conidiation characteristics of said ATCC No. 6275 which are negative in the presence of FK-506/rapamycin type immunosuppressant activity.
A process for testing a compound or fermentation broth for FK-506/rapamycin type or cyclosporin A type immunosuppressant activity comprising the steps of:
- (1) contacting said compound or broth with (a) Aspergillus niger ATCC No. 74085 mutant (b) Aspergillus niger ATCC No. 74086 mutant;
- (c) Aspergillus niger ATCC No. 74087 mutant;
- (d) Aspergillus niger ATCC No. 6275;
- and (2) observing the conidiation characteristics of said mutants, which is:
- positive for ATCC No. 74086 and ATCC No. 74087, but negative for ATCC No. 74085 and ATCC No. 6275 in the presence of FK-506/rapamycin immunosuppressant-type activity;
- and positive for ATCC No. 74085 and ATCC No. 74086, but negative in the presence of ATCC No. 74087 and ATCC No. 6275 in the presence of cyclosporin A immunosuppressant-type activity.
Dependencies are inferred from the claim text.