Cloning and expression of a variant gene of platelet factor 4 and compositions thereof to modulate immune responses
AssigneeSRI International
InventorsChristopher J. Green, Paul H. Johnson
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Abstract
The present invention provides for the purification from native sources or the cloning and expression of a variant form of platelet factor 4 and also provides recombinant DNA vectors and methods for the expression and recovery of the platelet factor 4 variant. Also provided are compositions and methods for modulating immune responses in mammals comprising immunomodulating effective amounts of platelet factor 4 variant.
Claims5
A substantially pure, isolated protein encoded by the nucleic acid sequence of platelet factor 4 variant 1.
A composition for modulating immune response in a mammal comprising an effective amount for modulating immune response of platelet factor 4 variant 1, and a pharmaceutically acceptable carrier.
Platelet factor 4 variant 1 produced by the method which comprises:
- a) culturing E. coli cells transformed with a plasmid expression vector comprising an isolated, cloned recombinant or synthetic nucleic acid sequence encoding PF4var1 protein or a precursor form of PF4var1 protein operably linked to a control sequence for expression ligated in translational reading phase with a second nucleic acid sequence to form a fused gene;
- b) disrupting the transformants;
- c) purifying the fusion protein from other cellular proteins;
- d) cleaving the fusion protein at the specific cleavage site;
- and
- e) recovering the platelet factor 4 variant from the cleaved product of step (d).
A substantially pure, isolated protein encoded by an isolated, cloned recombinant or synthetic nucleic acid encoding PF4var1 protein or a precursor form of PF4var1 protein.
Dependencies are inferred from the claim text.