Single-chain hepatocyte growth factor variants
AssigneeGenentech, Inc.
InventorsPaul J. Godowski, Natalie A. Lokker, Melanie R. Mark
Life of this patent
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Abstract
The invention concerns hepatocyte growth factor (HGF) variants that are resistant to proteolytic cleavage by enzymes capable of in vivo conversion of HGF into its two-chain form. The single-chain HGF variants, which preferably have an amino acid alteration at or adjacent to any of amino acid positions 493, 494, 495 and 496 of the wild-type human HGF sequence, retain their ability to bind the HGF receptor.
Claims25
An isolated nucleotide molecule encloding a hepatocyte growth factor (HGF) variant stabilized in single-chain form by mutagenesis within a region recognized by an enzyme capable of converting HGF into its two-chain form, said variant being capable of binding an HGF receptor and substantially lacking the ability to activate said receptor.
An isolated nucleotide molecule encoding a human HGF (huHGF) amino acid sequence variant stabilized in single-chain form by an amino acid alteration at or adjacent to amino acid positions 493, 494, 495 or 496 of the wild-type huHGF receptor and substantially lacking the ability to activate said receptor.
Dependencies are inferred from the claim text.