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US 5,316,919 AGranted
Method of producing 2 KD to 10 KD peptides having no L-methionine residue
OwnerAkira Kajiinventor
Life of this patent
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Abstract
Novel peptides having pharmacologic activities, such as vasodilating, hypotensive and bronchodilating activities, and a method of making peptides are described.
Claims4
A method of producing a peptide having no L-methionine residue and having a molecular weight of 2,000 to 10,000, comprising;
- (A) cultivating prokaryotic cells carrying a recombinant pUC plasmid which contains a trp promoter and a DNA coding for a fused protein, said fused protein comprising;
- (1) a galK protein fragment comprising 200 to 300 amino acid residues from the N terminus of the galK protein, and
- (2) an L-methionine-free peptide having a molecular weight of 2,000 to 10,000 coupled to said galK protein fragment at the C terminus thereof via an L-methionine residue;
- to cause expression of said fused protein;
- (B) purifying said fused protein;
- (C) cleaving said purified fused protein with cyanogen bromide to yield a peptide mixture;
- and
- (D) isolating peptides which have a molecular weight of 2,000 to 10,000 from the peptide mixture to obtain said peptide having no L-methionine residue.
5 more elements
A recombinant pUC plasmid comprising a trp promoter and a DNA coding for a fused protein, said fused protein comprising:
- (1) a galK protein fragment comprising 200 to 300 amino acid residues from the N terminus of galK;
- and
- (2) an L-methionine-free peptide having a molecular weight of 2,000 to 10,000 coupled to said galK protein fragment at the C terminus thereof via an L-methionine residue.
Dependencies are inferred from the claim text.