Recombinant 40 KDa Dermatophagoides farinae allergen
AssigneesFumakilla Limited, Hiroshima University
InventorsSatoru Oka, Kazuhisa Ono, Seiko Shigeta, Takeshi Wada
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Abstract
The present invention is directed to a recombinant mite allergen obtainable by expression of a mite-body-derived gene, a gene which codes for said allergen, a mite allergen fragment, a polypeptide having an epitope contained in said allergen, an expression vector capable of expressing the gene, a bacterium, yeast or mammalian cell transformed with said expression vector, a method for producing said allergen, and a pharmaceutical composition or a diagnostic reagent for the treatment of mite allergic diseases.
Claims16
A substantially pure recombinant mite allergen obtainable by expression of a mite-body-derived gene fragment, which contains the partial amino acid sequence shown in SEQ ID NO:2:
- ##STR13##
A substantially pure recombinant mite allergen obtainable by expression of a mite-body-derived gene fragment, which contains the partial amino acid sequence shown in SEQ ID NO:4:
- ##STR14##
A substantially pure mite allergen fragment containing at least an amino acid sequence encoded in the region of about 170 bp to about 270 bp, about 270 bp to about 400 bp, or about 170 bp to about 400 bp from the translation start in the restriction map shown in FIG. 10.
A substantially pure polypeptide having an epitope contained in an amino acid sequence encoded by a DNA fragment as shown in the restriction map shown in FIG. 10, said DNA fragment being a cDNA obtained from Dermatofagoides farinae, said DNA fragment being selected from the group consisting of 170 bp to 270 bp from the start of translation, 270 bp to 400 bp from the start of translation and 170 to 400 bp from the start of translation.
A substantially pure recombinant mite allergen, wherein said recombinant mite allergen has the amino acid sequence shown in SEQ. ID. No. 1 and is produced by a process comprising the steps of:
- (a) expressing a fusion gene composed of a mite-body-derived gene ligated to a gene encoding at least a second protein;
- (b) collecting a fusion protein composed of a mite-body-derived allergen and said second protein obtained from step (a);
- (c) separating said second protein from said mite-body-derived allergen;
- (d) collecting said mite-body-derived allergen.
A substantially pure recombinant mite allergen, wherein said recombinant mite allergen has the amino acid sequence shown in SEQ. ID. No. 2 and is produced by a process comprising the steps of:
- (a) expressing a fusion gene composed of a mite-body-derived gene ligated to a gene encoding at least a second protein;
- (b) collecting a fusion protein composed of a mite-body-derived allergen and said second protein obtained from step (a);
- (c) separating said second protein from said mite-body-derived allergen;
- (d) collecting said mite-body-derived allergen.
Dependencies are inferred from the claim text.