Protein having nitrile hydratase activity obtained from rhizobium, gene encoding the same, and a method for producing amides from nitriles via a transformant containing the gene

Abstract

A novel water-soluble enzyme protein being composed of three heterogeneous subunits and having nitrile hydratase activity was separated and purified from the bacterium of genus Rhizobium. The nucleotide sequence of the gene encoding the protein was determined; using the transformant transformed with the expression vector having the nucleotide sequence, the protein was expressed; by reacting the transformant with a nitrile in a culture broth, the corresponding amide was produced. Via the use of a culture broth containing the transformant described above or the protein itself, a nitrile can be efficiently converted into the corresponding amide.

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