Drosophila cell lines expressing genes encoding MHC class I antigens and B2-microglobulin and capable of assembling empty complexes and methods of making said cell lines
AssigneeThe Scripps Research Institute
InventorsPer A. Peterson, Michael Jackson, Pierre Langlade-Demoyen
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Abstract
The present invention relates to a rational, elegant means of producing, loading and using Class I molecules to specifically activate CD8 cells in vitro, and their therapeutic applications in the treatment of a variety of conditions, including cancer, tumors or neoplasias, as well as viral, retroviral, autoimmune, and autoimmune-type diseases. The present invention also relates to vectors, cell lines, recombinant DNA molecules encoding human .beta.2 microglobulin or Class I MHC molecules in soluble and insoluble form, and methods of producing same.
Claims9
A stable Drosophila cell line comprising:
- a human class I MHC gene, operably linked to an inducible promoter, capable of expressing a human class I MHC antigen on induction of said promoter;
- a human .beta.-2-microglobulin gene, operably linked to a second inducible promoter, capable of expressing a human .beta.-2-microglobulin protein, on induction of said second promoter;
- wherein said cell is capable of assembling said MHC class I antigen and said .beta.-2-microglobulin protein into empty complexes, and transporting and anchoring said empty complexes to the surface of the cell in sufficient numbers to activate a population of T-cell lymphocytes against a selected peptide when said peptide is bound to said complexes.
Dependencies are inferred from the claim text.