Fructose transferring enzyme absorbed on a granular carrier for production of fructooligosaccharides
AssigneeMeiji Seika Kaisha, Ltd.
InventorsToshiaki Kono, Goichi Yamaguchi, Hidemasa Hidaka
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Abstract
A fructose transferring enzyme is immobilized by adsorption on a granular carrier having a primary to quaternary amine. The carrier is preferably an epoxy polymer, a vinyl polymer or a chitosan derivative having a primary, secondary or tertiary amine. Immobilization can be performed without or with a crosslinking agent. The immobilized enzyme is used for producing fructooligosaccharides by passing a sucrose solution through a column containing the immobilized enzyme.
Claims21
An immobilized enzyme adsorbed to a granular carrier, said immobilized enzyme being produced by a process consisting essentially of the step of immobilizing free enzyme having fructose transferring activity by adsorbing the free enzyme onto the granular carrier, said immobilizing step being carried out without the use of a crosslinking agent, wherein said carrier is selected from the group consisting of epoxy polymers, vinyl polymers and chitosan derivatives, each containing at least one primary, secondary, or tertiary amine.
A process for producing an immobilized enzyme adsorbed to a granular carrier, said process consisting essentially of the step of immobilizing free enzyme having fructose transferring activity by adsorbing the free enzyme onto the granular carrier, said immobilizing step being carried out without the use of a crosslinking agent, wherein said carrier is selected from the group consisting of epoxy polymers, vinyl polymers and chitosan derivatives, each containing at least one primary, secondary or tertiary amine.
A process for producing a fructooligosaccharide, wherein said process comprises the step of contacting sucrose with an immobilized enzyme having fructose transferring activity, wherein said enzyme is immobilized by adsorbing free enzyme having fructose transferring activity onto a granular carrier, said immobilizing step being carried out without the use of a crosslinking agent, wherein said carrier is selected from the group consisting of epoxy polymers, vinyl polymers and chitosan derivatives, each containing at least one primary, secondary, or tertiary amine.
Dependencies are inferred from the claim text.