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AssigneesInstitut Pasteur, Institut Nationale de la Sante et de la Recherche Medicale
InventorsPierre Tiollais, Charles Chany, Marie-Francoise Dubois, Christine Pourcel, Anne Louise
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Method for the production of antigens vaccinating against the virus of B viral hepatitis. It consists of transforming a cell culture with a vector, more particularly a plasmid, itself containing an insertion sequence including itself at least the part of the viral DNA coding for the immunogen protein, capable of inducing in vivo antibody production active with respect to the whole virus, as well as the viral promoter under the control of which the transcription and translation of the above-said part of viral DNA is normally carried out, in particular in a host infected with the corresponding virus.
Genetically engineered spherical particles having sizes in the range of about 18 to about 25 nm, which particles are totally free of human components, are free of DNA polymerase, totally free of Dane particles, of HB.sub.e and HB.sub.c antigens, are agglutinated by antibodies which agglutinate HB.sub.s Ag of human serum, are not detected by anti-HB.sub.c antibodies and by anti-HB.sub.e antibodies, which particles are immunogenic and have vaccinating properties against hepatitis B virus, and are incapable of causing infectious hepatitis.
Genetically engineered antigenic spherical particles of a size in the range of about 18 to about 25 nm, which particles are free of DNA polymerase, totally free of Dane particles, totally free of human components, of HB.sub.e and HB.sub.c antigens, are agglutinated by antibodies which agglutinate HB.sub.s Ag of human serum, are not detected by anti-HB.sub.c antibodies and by anti-HB.sub.e antibodies, which particles are immunogenic and have vaccinating properties against hepatitis B virus, which particles do not elicit an autoimmune response in a treated patient and are incapable of causing infectious hepatitis.
Eucaryotically genetically engineered antigen spherical particles of a size in the range of about 18 to about 25 nm which particles are free of DNA polymerase, totally free of Dane particles, totally free of human components, of HB.sub.e and HB.sub.c antigens, are agglutinated by antibodies which agglutinate HB.sub.s Ag of human serum, are not detected by anti-HB.sub.c antibodies and by anti-HB.sub.e antibodies, which particles are immunogenic and have vaccinating properties against hepatitis B virus, which particles do not elicit an autoimmune response in a treated patient and are incapable of causing infectious hepatitis.
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