Process and test carrier for the determination of an enzyme from an isoenzyme mixture
AssigneeBoehringer Mannheim GmbH
InventorsHans-Erich Wilk, Anselm Rothe, Erich Schneider
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Abstract
The present invention provides a process for the determination of an enzyme from an isoenzyme mixture in a liquid sample by inhibition of the disturbing isoenzymes and determination of the non-inhibited enzyme, wherein the isoenzyme mixture is contacted with one or more substances which are able to inhibit the disturbing isoenzymes, the sample containing the inhibiting substance(s) is transferred to a small-pored reaction medium and the disturbing enzyme is there inhibited and the determination of the non-inhibited enzyme is carried out in the resulting liquid. The present invention also provides a test carrier for carrying out this process.
Claims20
A process for the determination of a specific isoenzyme present in an isoenzyme mixture such as a body fluid sample, wherein any other isoenzymes present in the mixture are inhibited, and the specific isoenzyme is determined in less than five minutes, with no requisite step for removal of the inhibited isoenzymes, comprising
- contacting the sample with one or more substances which bind to and are capable of inhibiting all but the specific isoenzyme so that the entire sample remains soluble,
- transferring the entire sample to a small-pored reaction medium with a pore size less than 25 .mu.m,
- inhibiting all but the specific isoenzyme while maintaining the entire sample in solution, and
- carrying out the determination of the specific isoenzyme in the presence of the inhibited isoenzymes which remain in solution.
A flow through test carrier for the determination of a specific isoenzyme present in an isoenzyme mixture in a liquid sample consisting of:
- a sample application region and an evaluation region and at least one test layer, wherein
- the sample application region comprises a large-pored material (A) having an air passage of more than 2000 l/m.sup.2 sec.sup.2 and containing one or more substances which bind and inhibit all other isoenzymes without immobilizing them, and a small pored reaction medium (B) with a pore size less than 25 .mu.m, in direct contact with A which facilitates transport of a liquid sample from A to B, where the ratio of pore size A:B is in the range of 2:1 to 2,000:1;
- and wherein
- the evaluation region (C) comprises at least one layer containing the test substances necessary for the determination of the specific isoenzyme in the presence of the inhibited, non-immobilized isoenzymes by means of a characteristic test signal and wherein C is in direct contact or can be placed into such contact with B to permit transfer of the liquid sample from B to C.
Dependencies are inferred from the claim text.