Probes to Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis
AssigneeBecton, Dickinson and Company
InventorsColleen M. Nycz, James L. Schram, Daryl D. Shank, Glenn P. Vonk
Life of this patent
Estimating the term…
Abstract
The invention provides methods and nucleic acid probes for deleting, amplifying, and isolating sequences of Mycobacterium avium, Mycobacterium intracellulare, and Mycobacterium paratuberculosis.
Claims24
An oligonucleotide consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or ribonucleic acid forms thereof.
The oligonucleotide of claims 1 through 16 which is the ribonucleic acid form thereof.
A method of amplifying Mycobacterium avium, Mycobacterium intracellulare, or Mycobacterium paratuberculosis nucleic acid sequences comprising hybridizing an oligonucleotide primer consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or ribonucleic acid forms thereof to Mycobacterium nucleic acids in a sample and amplifying the Mycobacterium nucleic acid sequences by extension of the primer.
A method of detecting Mycobacterium avium, Mycobacterium intracellulare, or Mycobacterium paratuberculosis nucleic acid sequences which comprises hybridizing an oligonucleotide probe consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or ribonucleic acid forms thereof to Mycobacterium nucleic acid sequences in a sample and detecting hybridization by means of a detectable marker included in or conjugated to the probe.
A method of amplifying Mycobacterium avium, Mycobacterium intracellulare, or Mycobacterium paratuberculosis nucleic acid sequences and detecting the amplified product which comprises hybridizing an oligonucleotide primer consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15, or ribonucleic acid forms thereof to Mycobacterium nucleic acid sequences in a sample, amplifying the nucleic acid sequences by extension of the primer, hybridizing a probe consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, SEQ ID NO:15 to the amplified nucleic acid sequences and detecting hybridization by means of a detectable marker included in or conjugated to the probe.
A kit comprising an oligonucleotide primer or probe consisting of SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:
- 9, SEQ ID NO:
- 10, SEQ ID NO:11, SEQ ID NO:12, SEQ ID NO:13, SEQ ID NO:14, or SEQ ID NO:15, or ribonucleic acid forms thereof and reagents for use with the primer or probe for amplification or detection of Mycobacterium nucleic acid sequences in a sample.
Dependencies are inferred from the claim text.