Capillary tube with reversible protein interaction and method
AssigneeHewlett-Packard Company
InventorSally A. Swedberg
Life of this patent
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Abstract
A method for treating capillary walls exposable to protein solutes is provided that promotes reversible interactions between the protein solutes and the wall. Thus a small bore capillary tube, useful for reproducible protein electrophoretic separations, includes an interfacial phase comprising agarose that is bonded to the bore. Pretreatment of the capillary walls with silylation reagents forms an intermediate layer that provides a determinable cathodic electroosmotic flow magnitude that remains constant even if the buffer pH fluctuates between 4-7. The modified capillaries can be used repeatedly and they exhibit selectivity in protein separations.
Claims11
A device for electrophoretic separations of protein solutes, comprising:
- a capillary tube that has an inner wall;
- reservoir and injection means for holding analyte that includes the protein solutes and for injecting the analyte into the capillary tube;
- power supply means for applying an electric field along the capillary tube;
- detection means for detecting separated solutes within the capillary tube;
- and
- means for providing reversible interactions between the protein solutes and the inner wall and including an interfacial layer, which includes agarose and is bonded coavalently to the inner wall.
A device for electrophoretic separations of protein solutes, comprising:
- a capillary tube that has an inner wall;
- an interfacial layer bonded to the inner wall;
- reservoir and injection means for holding analyte that includes the protein solutes and for injecting the analyte into the capillary tube;
- power supply means for applying an electric field along the capillary tube;
- detection means for detecting separated solutes within the capillary tube;
- and
- an outer layer including agarose that is covalently bonded to the interfacial layer;
- whereby interactions between the protein solutes and the inner wall are reversible.
A method for treating a solid silica inner wall surface of an electrophoresis capillary exposable to protein solutes comprising the steps of:
- depositing an interfacial layer on the inner wall surface;
- and
- depositing, by covalently bonding, an outer layer containing agarose on the interfacial layer;
- whereby interactions between the protein solutes and the inner wall are reversible.
A coated capillary in a capillary electrophoresis apparatus for electrophoretic separations of protein solutes, comprising:
- a capillary tube that has an inner wall;
- an interfacial layer bonded to the inner wall;
- and
- an outer layer including agarose that is coavalently bonded to the interfacial layer;
- whereby interactions between the protein solutes and the inner wall are reversible.
Dependencies are inferred from the claim text.