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US 5,314,542 AGranted
Nitrosomonas preservation and reactivation for aquaria
AssigneePrecision Aquarium Testing Inc.
InventorsEdward J. Cassidy, Ronald D. Jones
Life of this patent
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Abstract
A culture of Nitrosomonas packaged in a manner to induce a metabolic state of dormancy under conditions favorable for survival of up to at least one year at room temperature: with a method for its rapid reactivation to complete metabolic activity within about 72 hours: for its subsequent addition into aquaria to immediately begin the oxidation and prevention of harmful ammonia accumulation.
Claims1
A method for reactivating a metabolic activity of liquid culture of dormant ammonia oxidizing bacteria prior to their addition into an aquarium, comprising the steps of:
- a. increasing a concentration of ammonia in said liquid culture to about 200 ppm, and
- b. waiting about 72 hours to restore the metabolic activity of said dormant ammonia oxidizing bacteria before adding said culture into said aquarium.
Dependencies are inferred from the claim text.