Method and means for density gradient centrifugation
AssigneeEldan Technologies Co. Ltd.
InventorsMichael Inbar, Gerald Slutzky, Mordechai Radushetzky, David Shitrit
Life of this patent
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Abstract
In the performance of density gradient centrifugation back mixing after centrifugation is prevented by placing a snugly fitted layering insert within the vessel. The insert is capable of supporting the body of liquid dispersion to be centrifuged under regular gravity condition and enables bi-directional cross-flow during centrifugation. A small mesh filter plate may be placed on top of the layering insert.
Claims13
A method of performing density gradient centrifugation for the recovery of at least one solid matter component from a liquid dispersion, comprising providing a centrifugation vessel having a lower part and an upper part, charging a body of working fluid into the lower part and a body of said liquid dispersion into the upper part of the centrifugation vessel, sealing the vessel and subjecting it to centrifugation whereby a density gradient is established, and withdrawing said at least one solid matter component, wherein a snugly fitting layering insert is provided within said centrifugation vessel for supporting said body of liquid dispersion under regular gravity conditions and enabling bi-directional cross-flow during centrifugation, and wherein the insert comprises a disk having one or more narrow cuts or lines of weakness whereby the disk is divided into segments which yield under the action of centrifugal forces.
A layering insert for use in density gradient centrifugation, which layering insert fits snugly into a centrifugation vessel for supporting a body of liquid dispersion inside said centrifugation vessel under regular gravity conditions and enables bi-direction cross-flow during centrifugation, the insert comprising a disk having one or more narrow cuts or lines of weakness whereby the disk is divided into segments which yield and collapse under the action of centrifugal forces.
A centrifugation vessel comprising a layering insert and holding a body of working fluid underneath the layering insert, said layering insert being fitted snugly into the centrifugation vessel for supporting a body of liquid dispersion inside said centrifugation vessel under regular gravity conditions and enabling bi-direction cross-flow during centrifugation, and a small mesh filter plate super-positioned on said layering insert.
Dependencies are inferred from the claim text.