Recombinant DNA encoding neutral trehalase
AssigneeGist-Brocades N.V.
InventorsMarianne Driessen, Klaas A. Osinga, Margareta A. Herweijer
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Abstract
A transformed yeast is disclosed, comprising at least one gene encoding neutral trehalase or trehalose-6-phosphate synthase which gene has been modified such that it differs from a corresponding wild-type gene encoding neutral trehalase or trehalose-6-phosphate synthase, the yeast having as a result a different trehalose content from the untransformed parent yeast. The sugar resistance and drying resistance of the yeast compared to those of the untransformed strain are thereby improved.
Claims1
A recombinant DNA comprising a gene or part of a gene coding for neutral trehalase of yeast selected from the group consisting of
- i) the NDA insert 16.1.1 in plasmid pTRE16.1.1 (CBS 115.91),
- ii) A DNA capable of hybridizing under stringent conditions to a DNA of i) and encoding a protein having the enzymatic activity of the protein encoded by i) and
- iii) A DNA encoding the same amino acid sequence as that of i) or ii).
Dependencies are inferred from the claim text.