Fractionation of polyalkylene oxide-conjugated hemoglobin solutions
AssigneeEnzon, Inc.
InventorsRobert G. L. Shorr, Myung-Ok P. Cho, Kwang Nho
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Abstract
Hemoglobin-containing solutions containing polyalkylene oxide-conjugated hemoglobin having a molecular weight greater than about 85,000 daltons and a degree of substitution of at least five polyalkylene oxide conjugates per hemoglobin molecule are described that are not associated with hemoglobinuria in mammals. A method of simultaneously fractionating and purifying polyalkylene oxide-conjugated hemoglobins is also disclosed.
Claims21
A polyalkylene oxide-conjugated hemoglobin-containing solution comprising polyalkylene oxide-conjugated hemoglobin in a physiologically acceptable aqueous buffer solution, said polyalkylene oxide-conjugated hemoglobin having a molecular weight greater than about 85,000 daltons and a degree of substitution of at least five polyalkylene oxide conjugates per hemoglobin molecule, wherein said solution does not produce hemoglobinuria in mammals.
The solution of claim wherein said hemoglobin comprises a hemoglobin produced by recombinant methods.
Dependencies are inferred from the claim text.