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US 5,312,763 AGranted
Method for the detection of analytes
AssigneeBoehringer Mannheim GmbH
InventorsHorst Baumgarten, Michael Grol, Peter Stahl
Life of this patent
Estimating the term…
Abstract
In order to detect analytes with an immunological method by incubation with receptors and analysis of the complexes formed, a mixture of at least two monoclonal antibodies which are specifically bindable to the analyte and whose cross-reactivities are different are used for at least one of the receptors.
Claims6
Method for determining a monovalent analyte in a sample comprising
- combining a sample to be analyzed with (i) a known amount of labelled or immobilized monovalent analyte to be detected, and (ii) a mixture of at least two different monoclonal antibodies which specifically bind to said monovalent analyte, wherein each monoclonal antibody in said mixture exhibits different cross reactivity with substances other than said monovalent analyte, and
- determining binding to said monoclonal antibodies to said labelled or immobilized analyte to determine said analyte in said sample.
Method for determining a monovalent analyte in a sample, comprising
- combining a sample to be analyzed with (i) a known amount of said monovalent analyte bound to a solid phase, and (ii) a mixture of at least two labelled monoclonal antibodies which specifically bind to said monovalent analyte, wherein each monovalent antibody in said mixture exhibits different cross reactivity with substances other than said monovalent analyte, and
- determining binding of said labelled monoclonal antibodies to said solid phase monovalent analyte to determine analyte in said sample.
Dependencies are inferred from the claim text.