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US 5,312,759 AGranted
Method for measurement of fructosamines using 1,2-quinones
AssigneeIatron Laboratories, Inc.
InventorsMichio Hama, Michiyo Nakayama, Mitsunao Tanaka
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Abstract
A method for measurement of fructosamines in an aqueous liquid characterized by including the steps of: (a) bringing an aqueous liquid sample into contact with one or more active oxygen producing substances; (b) bringing the product of said step (a) into contact with a reagent producing a detectable change in the presence of hydrogen peroxide derived from the active oxygen, and (c) detecting an occurrence of the detectable change of said step (b).
Claims6
A method for measurement of fructosamines in an aqueous liquid which comprises the steps of:
- (a) bringing an aqueous liquid sample into contact with a 1,2-quinone compound of the general formula:
- ##STR8## wherein, B.sub.3 and B.sub.4 are respectively hydrogen atoms or are atoms necessary for forming a benzene ring or a pyridine ring together with the two carbon atoms in the formula, and B.sub.5 and B.sub.6 are atoms necessary for forming a benzene ring or a pyrrole ring together with the two carbon atoms in the formula, said pyridine being substituted by carboxylic acid groups and said pyrrole ring being substituted by a carboxylic acid group, at a pH of from 8.5 to 11;
- (b) bringing the product of step (a) into contact with a reagent for measuring hydrogen peroxide for measuring hydrogen peroxide resulting from the reaction of step (a) between said 1,2-quinone compound and said fructosamines;
- and
- (c) measuring a detectable change in the reagent of step (b).
2 more elements
Dependencies are inferred from the claim text.