Method of immobilizing and preserving an immunologically reactive substance

A method for immobilizing and preserving an immunologically reactive antigen substance on a surface of a solid support comprising:

• (a) centrifugally bringing a suspension of immunologically reactive antigen substance selected from the group consisting of antigenic cells and non-cell bound into contact with a surface of a solid support having physical adsorption capability so as to permit the antigen substance to be immobilized on the solid support by means of physical adsorption;
• (b) bringing a 1.0 to 5.0% formaldehyde solution or a 0.003 to 0.06% glutaraldehyde solution into contact with the antigen substance immobilized by physical adsorption on the solid support so as to more strongly bond the antigen substance on the solid support while maintaining the antigenicity of the immobilized antigen substance;
• (c) washing the solid support with a washing solution capable of inhibiting further action performed by the formaldehyde or the glutaraldehyde;
• and
• (d) contacting the immobilized antigen substance on the solid support with a protecting solution itself containing 0.5 to 10.0% of sugar and a suitable amount of antiseptic substance so as to protect the antigen substance immobilized on the solid support from structural damage.