Process for producing taxol by cell culture of taxus species
AssigneeNippon Steel Corporation
InventorsKoji Saito, Hiroaki Ohashi, Masaaki Hibi, Makoto Tahara
Life of this patent
Estimating the term…
Abstract
A callus and suspension culture cells containing taxol as an effective component, and a process for producing taxol therefrom, are provided. The callus and suspension culture cells containing taxol in a high content can be induced and proliferated by culturing a tissue of a plant belonging to the genus Taxus, particularly its female gametophyte. The process for cell culture is advantageously carried out in the presence of a particular combination of plant growth regulators known per se in a growth medium. A callus or suspension culture cells containing at least taxol as an effective component can be stably provided, making it possible to effectively produce taxol.
Claims9
A process for producing taxol by culturing cells originating from tissues of plants belonging to Taxus cuspidata SIEB. et ZUCC which comprises:
- a) preparing a living tissue of Taxus cuspidata
- b) culturing the tissue obtained in step a) in a nutrient medium containing gibberellin, suitable for inducing a callus, to thereby induce a callus;
- c) culturing the callus cells obtained in step b) in a nutrient medium suitable for proliferating suspension culture cells;
- and
- d) recovering taxol from the cultured products obtained in step c).
A callus which contains at least taxol as alkaloids, and obtained by a process comprising the steps of:
- a) preparing a leaf, shoot or female gametophyte of Taxus cuspidata;
- and
- b) culturing the leaf, shoot or female gametophyte obtained in step a) in a medium containing gibberellin suitable for inducing a callus, to thereby induce a callus.
Suspension culture cells which contain at least taxol as alkaloids, and obtained by a process comprising the steps of:
- a) preparing a leaf, shoot or female gametophyte of Taxus cuspidata;
- b) culturing the leaf, shoot or female gametophyte obtained in step a) in a medium containing gibberellin suitable for inducing a callus, to thereby induce a callus;
- and
- c) culturing the cells obtained in step b) in a nutrient medium suitable for proliferating suspension culture cells.
Dependencies are inferred from the claim text.