Production of high-maltose syrup and high-protein byproduct from materials that contain starch and protein by enzymatic process
AssigneeNational Science Council
InventorJei-Fu Shaw
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Abstract
An enzymatic process is developed to produce high-maltose syrup and high-protein byproduct simultaneously from materials that contain starch and protein. In the process, the slurry of milled starting material is first liquefied with .alpha.-amylase and then centrifuged. The precipitated fraction is recovered as high-protein flour. The supernatant fraction was then saccharified with .beta.-amylase and debranching enzyme (isoamylase or pullulanase) simultaneously to produce high-maltose syrup at various conditions. The yield of high-maltose syrup is affected by temperature, pH, DE values of liquefied starch, different enzyme combinations and varieties of starting materials.
Claims7
An enzymatic process for producing a high-maltose syrup and a high-protein byproduct from a material that contains starch and protein, said process comprising the steps of:
- liquefying starch in said material in an autoclave using an effective amount of .alpha.-amylase to obtain a soluble starch hydrolysate and heat-coagulated protein;
- separating said heat-coagulated protein from said soluble starch hydrolysate;
- and
- saccharifying said soluble starch hydrolysate using an effective amount of .beta.-amylase and debranching enzymes comprising a combination of isoamylase and pullulanase.
Dependencies are inferred from the claim text.