CKS method of HCV protein synthesis
AssigneeAbbott Laboratories
InventorsTimothy J. Bolling, Wlodzimierz Mandecki
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Abstract
Disclosed is a method of producing fusion proteins, particularly HCV fusion proteins, wherein one part of the fusion protein is formed from the bacterial protein CKS.
Claims14
A method for expressing HCV protein in a prokaryotic cell, said method comprising the steps of:
- (a) providing a DNA vector having:
- 1) a control region, said control region comprising a prokaryotic promoter and a prokaryotic binding site, wherein said control region directs expression of a DNA sequence comprising two elements operatively linked in a 5' to 3' direction, a first element encoding CKS protein;
- and
- 2) a second element encoding said HCV protein, wherein said two elements are contiguous and in the same reading frame;
- (b) transforming said prokaryotic cell with said DNA vector;
- and
- (c) expressing a fusion protein of CKS protein and said HCV protein.
A cloning vector for transforming cells to express heterologous HCV protein, said cloning vector comprising a plasmid having a prokaryotic control region comprising a prokaryotic promoter and a prokaryotic ribosome binding site, wherein said control region directs expression of a DNA sequence comprising two elements operatively linked in a 5' to 3' direction, a first element and a second element encoding CKS protein and said HCV protein to be expressed, wherein said two elements are contiguous and in the same reading frame.
A gene sequence for insertion into a plasmid vector, said gene sequence comprising in a 5' to 3' direction:
- a) a prokaryotic promoter;
- b) a prokaryotic ribosome binding site;
- c) a first gene fragment encoding CKS protein;
- and
- d) a second gene fragment encoding HCV protein to be expressed, wherein said first and second gene fragments are contiguous and in the same reading frame.
Dependencies are inferred from the claim text.