Supersecreting mutants of saccharomyces cerevisiae
AssigneeCollaborative Research, Inc.
InventorsGerald R. Fink, Catherine M. Buckley
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Abstract
This invention relates to the production of useful protein products by secretion from yeast cells. In particular, it relates to the identification of supersecreting mutant yeast cells, isolation of the wild-type gene which complements the mutation, and construction of non-leaky, non-reverting supersecreting strains.
Claims3
A supersecreting saccharomyces cerevisiae yeast cell exhibiting at least two-fold increased translocation of a heterologous protein through its secretory pathway and having an SSC1 gene which is disrupted or deleted making said gene incapable of producing a functional protein and rendering the supersecretion properties of said cell non-leaky and non-reverting.
A method of obtaining protein products from an SSC1 yeast cell strain capable of secreting a heterologous protein, said method comprising selecting said cell strain to have the SSC1 gene of FIGS. 5A-H modified by deletion of all codons distal to the EcoRI site which begins at nucleotide 1613 and culturing said strain to secrete said protein products.
A process of using a supersecreting yeast strain to obtain heterologous protein products, said process comprising culturing under conditions suitable for expression of said heterologous protein products a Saccharomyces cerevisiae yeast cell in which the SCC1 gene has been either a) disrupted with at least one other gene or b) partially deleted such that the resulting cell exhibits at least 2-fold translocation of said heterologous gene products through its secretory pathway and the supersecretion properties are non-reverting and non-leaky.
Dependencies are inferred from the claim text.