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US 5,312,730 AGranted
Immune complex transfer with lypophilic bridge
AssigneeCiba Corning Diagnostics Corp.
InventorsUri Piran, William J. Riordan, Deborah R. Silbert-Shostek
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Abstract
This invention relates to methods that have been found useful in reducing non-specific binding in immunochemical assays, via methods that can be implemented much faster than those used by Ishikawa. The techniques include the use of a lipophilic bridge, such as a liposome, or the elution of the antigen-antibody complex from the solid phase by the use of an anti-idiotypic antibody or an antibody, or the use of a heterologous reversible bridge.
Claims10
A rapid method for sensitive and accurate binding assay comprising
- a. reacting the analyte with
- 1. a labeled specific binder for the analyte and
- 2. a specific binder for the analyte which has been reversibly attached to a first solid phase via the use of a lipophilic material to form a labeled specific binder-analyte-specific binder-lipophilic material-first solid phase complex, wherein said lipophilic material provides a stable bridge between the solid phase and specific binder, and is readily dispersed by detergents or enzymes,
- b. separating the labeled specific binder-analyte-specific binder complex from the first solid phase with said detergents or enzymes,
- c. reattaching the labeled specific binder-analyte-specific binder complex to a second specific binder, which binds to the analyte or another component of the immune complex and which is attached to a second solid phase, and
- d. determining the amount of the analyte present by measurement of the amount of the labeled specific binder attached to the second solid phase.
3 more elements
Dependencies are inferred from the claim text.