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US 5,312,729 AGranted
Method of assay normalized with non-antigen calibrator
AssigneeAres-Serono Research & Development Limited Partnership
InventorsGerald J. Allen, Madlyn D. Denyer, Grenville A. Robinson
Life of this patent
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Abstract
The present invention relates to assay techniques for detection of ligands in solution and to means for putting such techniques into effect. In particular it relates to an improved assay technique which provides a liquid calibrator for use in standard assays in circumstances where the ligand itself is rare, expensive, or difficult to prepare in a sufficiently pure or quantifiable form.
Claims20
In a method of assay for a ligand in a sample comprising
- (1) incubating in a first solution the sample, sequentially or simultaneously, with a first specific binding partner to the ligand and with a predetermined amount of reagent X, wherein reagent X comprises a second specific binding partner to said ligand, bound directly or indirectly to a detectable label, or a ligand analogue, bound directly or indirectly to a detectable label, thereby forming a complex between said first specific binding partner and reagent X, the amount of reagent X in the complex being a function of the amount of ligand in the sample;
- (ii) removing said first specific binding partner from the solution by binding said first specific binding partner to a first solid phase reagent and separating said first solid phase reagent from the solution;
- and
- (iii) determining, by means of said detectable label, the amount of reagent X bound to said first solid phase reagent, the improvement comprising normalizing the assay by comparison with a calibration assay, said calibration assay comprising
- (a) incubating in a sample free second solution a predetermined amount of said reagent X with a calibration reagent Y comprising a specific binding partner for said reagent X other than the ligand and which is stable in solution at working concentrations and temperatures, thereby forming a complex between reagent Y and reagent X in a predictable and reliable manner;
- (b) removing said calibration reagent Y from said second solution by binding said calibration reagent Y to a second solid phase reagent and separating said second solid phase reagent from the solution in the same manner as that used to remove the first specific binding partner in step (ii);
- and
- (c) determining, by means of said detectable label, the amount of reagent X bound to said second solid phase reagent.
5 more elements
In a method of assay for a ligand in a sample comprising
- (i) incubating in a first solution the sample, sequentially or simultaneously, with a hapten-labelled first specific binding partner to the ligand and with a predetermined amount of reagent X, wherein reagent X comprises a second specific binding partner to said ligand, bound directly or indirectly to a detectable label, or a ligand analogue, directly or indirectly to a detectable label, thereby forming a complex between said first specific binding partner and reagent X, the amount of reagent X in the complex being a function of the amount of ligand in the sample;
- (ii) removing said first specific binding partner from the solution by binding said first specific binding partner to a first solid phase reagent and separating said first solid phase reagent from the solution;
- and
- (iii) determining, by means of said first detectable label, the amount of reagent X bound to said first solid phase reagent, the improvement comprising normalizing the assay by comparison with a calibration assay, said calibration assay comprising
- (a) incubating in a sample free second solution a predetermined amount of calibration reagent Z which is other than the ligand and which comprises said hapten-label bound, directly or indirectly, to said detectable label and which is stable in solution at working concentrations and temperatures with a specific binding partner for said hapten, thereby forming a complex between reagent Z and its specific binding partner in a predictable and reliable manner;
- (b) removing said calibration reagent Z from said second solution by binding said calibration reagent Z to a second solid phase reagent and separating said second solid phase reagent from the solution in the same manner as that used to remove the first specific binding partner from solution in step (ii);
- and
- (c) determining, by means of said detectable label, the amount of detectable label bound to said second solid phase reagent.
5 more elements
Dependencies are inferred from the claim text.