US 5,312,622 A · Acaricidal compositions and process for preparing same

Abstract

The invention relates to acaricidal compositions containing as active ingredient: the fermentation broth or cell-free fermentation broth, of a concentrate of the fermentation broth or cell-free fermentation broth, or a solidified form of the fermentation broth or cell-free fermentation broth of one or two bacterium strains deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the identification Nos. 001083 and 001086 or of their mixed micropopulation, if desired, in a sterilized state, optionally together with the metabolism products arising during the fermentation and inutilized nutriments, as well as one or more solid and/or liquid carrier(s) (preferably sugar or a grist of natural minerals), or an inert solvent (e.g. water) and, if desired, with surface active (preferably anionic or nonionic emulsifying or dispersing) agents. The compositions according to the invention can be used for protecting Apis mellifera (honey bee) against mites, particularly Varroa jacobsoni.

Claims9

Dependent claims

A biologically pure culture of the bacillus strain deposited at the National Collection of Agricultural and Industrial Microorganisms, Budapest, Hungary under the identification No. 001083, which forms mat, greyish colonies of 1 to 2 mm in diameter on a simple nutrient agar and shows the following properties:

______________________________________ a) Gram:
positive, b) sporulation:
oval, c) motion:
positive, d) anaerobic growth:
positive, e) growth on bismuth sulfite agar:
+/-, f) growth on eosin-methylene blue agar:
+/-, g) growth in the presence of 7% sodium positive, chloride:
h) catalase:
positive, i) NO.sub.3 --NO.sub.2 :
negative, j) Voges-Proskauer test negative, k) indole:
negative, l) urease (kristensen):
negative, m) arginine-dihydrolase:
negative, n) lysine-decarboxylase:
negative, o) ornithine-decarboxylase:
negative, p) aesculin hydrolysis:
positive, q) starch hydrolysis:
negative, r) casein hydrolysis:
negative, s) lecithin hydrolysis:
negative, t) gelatine hydrolysis:
positive, u) ammonium citrate:
negative, u) acid formation on the Hungh-Leifson's oxidative, oxidation-fermentation nutrient medium:
v) gas formation, peptone-water glucose:
negative, x) acid formation (BSS) glucose:
positive, fructose:
positive, lactose:
negative, maltose:
negative, mannitol:
positive, rhamnose:
negative, saccharose:
positive xylose:
negative, (peptone water) arabinose:
negative, adonitol:
negative, z) ONPG:
positive. ______________________________________

31 more elements

Dependencies are inferred from the claim text.

Acaricidal compositions and process for preparing same

Life of this patent

Acaricidal compositions and process for preparing same

Life of this patent

Abstract

Claims9