US 5,312,380 A · Endothelial cell procurement and deposition kit

Abstract

The invention is an endothelial cell procurement and deposition kit for collecting fat from a patient, processing said fat to produce an endothelial cell deposition product, and depositing said product on the surface of a graft, all under sterile conditions established and maintained within the components of said kit comprised of: fat collection means for collecting subcutaneous fat from a patient; digestion means connectable to said fat collection means to maintain sterility during reception of said fat and for retaining said fat under sterile conditions during rinsing and digestion to produce a digested product; endothelial cell isolation means connectable to said digestion means for maintaining sterile conditions during reception of said digested product and for separating and isolating microvessel endothelial cells from said digested product to produce an endothelial cell product; cell deposition means connectable to said isolation means for maintaining sterile conditions during reception of said endothelial cell product and for depositing said cells on the surface of a graft to be implanted in a patient and facilitating implantation of said endothelial graft into a patient.

Claims5

Dependent claims

A fat collection device, comprising:

a chamber having a top and a bottom;
a stopper means for sealing the chamber, capable of being slidably raised and lowered with respect to said chamber;
an inlet tube extending in sliding engagement through said stopper into said chamber;
a vacuum inlet tube extending in sliding engagement through said stopper means into said chamber;
a plunger means connected to said stopper means for lowering and raising said stopper means relative to the chamber, the inlet tube and the vacuum inlet tube;
a diaphragm means affixed to said stopper means for sealing said chamber during fat transfer, said diaphragm means being deflected by said inlet tube and said vacuum inlet tube when said stopper means is in a raised position and closing off both said inlet tube and said vacuum inlet tube when the stopper means is in a lowered position, thereby facilitating fat transfer from the chamber;
and
an outlet disposed at the bottom of the chamber, said outlet including a valve for regulating the transfer of fat from the chamber.

5 more elements

Dependencies are inferred from the claim text.

Endothelial cell procurement and deposition kit

Life of this patent

Endothelial cell procurement and deposition kit

Life of this patent

Abstract

Claims5