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US 5,310,884 AGranted
LTD.sub.4 receptor and photoaffinity probe
AssigneeMerck Frosst Canada, Inc.
InventorsRobert Zamboni, Kathleen M. Metters
Life of this patent
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Abstract
The LTD.sub.4 receptor has been defined by radioligand binding studies as a member of the family of G-protein coupled receptors. A photoactivable azido derivative of LTD.sub.4 ([.sup.125 I]Azido-LTD.sub.4) has been synthesized for use as a photoaffinity probe. Photoactivation of ([.sup.125 I]Azido-LTD.sub.4 under equilibrium binding conditions revealed the selective radiolabeling of a 45 kDa protein in guinea-pig lung membranes, as visualized by SDS-PAGE and autoradiography.
Claims1
The LTD.sub.4 receptor, which is a 45 kD protein, as determined by SDS-PAGE, bound to [.sup.125 I]azido of the formula:
- ##STR2##
Dependencies are inferred from the claim text.