Somatotropins with alterations in the .alpha.-helix 3 region
AssigneeAmerican Cyanamid Company
InventorDeborah T. Chaleff
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Abstract
The present invention relates to somatotropin analogues with amino acid changes in the .alpha.-helix 3 regions of said somatotropins, changes in the .alpha.-helix 2 regions, combinations thereof plus combinations with other changes to the native amino acid sequence of somatotropins. The resulting analogues are stable for formulation in sustained release, formulations, while maintaining biological activity. Further, methods for conducting site-directed mutagenesis on DNA encoding proteins and/or polypeptides also are provided.
Claims10
A somatotropin in which the alpha-helix 3 region is mutated, thereby enhancing the aqueous solubility of said somatotropin over the aqueous solubility of the native alpha-helix 3 form of said somatotropin while maintaining biological activity in a sustained release formulation.
Dependencies are inferred from the claim text.