Purification of Mullerian inhibiting substance
AssigneeThe General Hospital Corporation
InventorsPatricia K. Donahoe, Richard C. Ragin, David T. MacLaughkin
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Abstract
The present invention discloses a method of purifying Mullerian Inhibiting Substance. The method takes advantage of immunoaffinity chromatography to achieve MIS at up to 95% purity. The method provides for a purified MIS product in which contaminating enzymes having MIS proteolytic activity or inhibitors of MIS antiproliferative activity are substantially removed by addition of an alkali metal halide solution or an alkaline earth metal halide solution prior to elution with an acid solution having a pH of between about 2.5 and 4.0.
Claims9
A method of purifying recombinant MIS from host cells capable of expressing recombinant MIS comprising
- (a) binding the recombinant MIS to an antibody-chromatography matrix, said antibody being specific to MIS,
- (b) substantially removing contaminating enzymes having MIS proteolytic activity or inhibitors of MIS antiproliferative activity by adding to the matrix an effective amount of an alkali metal halide solution wherein said solution contains an effective amount of chelating agent, and
- (c) recovering the recombinant MIS by eluting with an acid solution having a pH of between about 2.5 and 4.0.
Dependencies are inferred from the claim text.