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US 5,310,878 AGranted
Biosynthetic cerebrospinal fluid control and method of use
AssigneeBaxter Diagnostics Inc.
InventorKathryn D. Herring
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Abstract
A biosynthetic cerebrospinal fluid control and method of use. Additionally, this invention relates to the isolation and purification of stable liquid human prealbumin, a component in the biosynthetic cerebrospinal fluid control.
Claims5
A method for purifying prealbumin comprising:
- (a) diluting human serum with a first buffer;
- (b) extracting globulins, ceroplasm and albumin from said human serum of Step (a) after dilution using ion exchange chromatography resulting in at least one fraction containing prealbumin;
- (c) isolating said prealbumin containing fractions eluted from Step (b) by immunodiffusion;
- (d) pooling said prealbumin containing fractions of Step (c) and concentrating and buffer exchanging said resulting prealbumin containing pooled fraction with a second buffer;
- (e) removing albumin from said prealbumin containing pooled fraction of Step (d) affinity chromatography;
- (f) isolating the prealbumin containing fractions eluted from Step (e) by immunodiffusion;
- (g) pooling said prealbumin containing fractions of Step (e) and concentrating and buffer exchanging said resulting prealbumin containing pooled fraction of Step (f) with a third buffer;
- (h) removing globulins from said prealbumin containing pooled fraction of Step (g) by ion exchange chromatography;
- (i) pooling, concentrating and buffer exchanging said prealbumin containing fraction of Step (h) with a fourth buffer;
- (j) purifying said prealbumin containing fraction of Step (i) by gel filtration to remove any residual proteins;
- (k) isolating said purified prealbumin fractions from Step (j);
- and
- (l) pooling, concentrating and sterile filtering said purified prealbumin fractions of Step (k);
- wherein the method yield is greater than about 60% prealbumin.
11 more elements
Dependencies are inferred from the claim text.