Integrin .alpha. subunit cytoplasmic domain polypeptides and antibodies
AssigneeThe Scripps Research Institute
InventorsRichard N. Tamura, Vito Quaranta
Life of this patent
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Abstract
Diagnostic systems, methods, polypeptides and antibodies for detecting the presence of the cytoplasmic domain of the integrin .alpha..sub.6B or .alpha..sub.3B subunit in a body sample are disclosed
Claims11
An antibody molecule that immunoreacts with a polypeptide consisting of an amino acid residue sequence shown in SEQ ID NO 3 from residue 1068-1091.
An antibody molecule that immunoreacts with a polypeptide consisting of an amino acid residue sequence shown in SEQ ID NO 3 from residue 1045 to residue 1091.
An antibody molecule that immunoreacts with a polypeptide consisting of an amino acid residue sequence shown in SEQ ID NO 5 from residue 121 to residue 141.
An antibody molecule that immunoreacts with a polypeptide consisting of an amino acid residue sequence shown in SEQ ID NO 9 from residue 113 to residue 153.
A method for detecting the presence of antigen having the cytoplasmic domain of .alpha..sub.6B in a body sample comprising the steps of:
- a) admixing the body sample with a composition containing antibody molecules that immunoreact with the .alpha..sub.6B protein and with a polypeptide consisting essentially of an amino acid residue sequence shown in SEQ ID NO 3 from residue 1045 to residue 1091 to form an immunoreaction admixture;
- b) maintaining said immunoreaction admixture under immunoreaction conditions for a time period sufficient for said antibody molecules to immunoreact with any .alpha..sub.6B present in said body sample and form an immunoreaction complex;
- and
- c) detecting the presence of any immunoreaction complex formed in step (b) and thereby detecting the presence of said antigen in said body sample.
An isolated polypeptide comprising the .alpha..sub.6B cytoplasmic domain sequence which consists essentially of an amino acid residue sequence shown in SEQ ID NO 5 from residue 121 to residue 141.
An isolated polypeptide that comprising the .alpha..sub.3B cytoplasmic domain sequence which consists essentially of an amino acid residue sequence shown in SEQ ID NO 9 from residue 113 to residue 153.
Dependencies are inferred from the claim text.