Rifampicin resistant xanthan hyperproducing Xanthomonas campestris strain
AssigneesShin-Etsu Chemical Co., Ltd., Shin-Etsu Bio, Inc.
InventorsThomas J. Pollock, Linda Thorne
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Abstract
A method of increasing xanthan gum production, comprising culturing a Xanthomonas campestris strain having a xanthan-increasing modification in a culture medium, wherein the modification is selected from the group consisting of (1) a mutation causing rifampicin-resistance; (2) a mutation causing bacitracin-resistance; or (3) exogenous genetic information controlling the synthesis of xanthan; and separating xanthan from the culture medium, is provided along with specific DNA sequences and Xanthomonas campestris strains showing increased xanthan gum production.
Claims2
A method for isolating a strain of Xanthomonas campestris which produces xanthan gum at a higher level of production than parent strain NRRL-B1459 S-4L II comprising:
- a) selecting rifampicin resistant colonies from said parent strain by growing said strain on medium containing rifampicin;
- b) measuring the xanthan gum production levels of said selected rifampicin resistant colonies;
- and
- c) selecting those rifampicin resistant colonies which produce xanthan gum in excess to that produced by said parent strain.
A biologically pure culture of a Xanthomonas campestris strain produced from parent strain NRRL-B1459 S-4L II which biologically pure culture strain is rifampicin resistant and produces more xanthan than said parent strain, said biologically pure culture strain being obtained by the steps comprising:
- a) selecting rifampicin resistant colonies from said parent strain by growing said parent strain on medium containing rifampicin;
- b) measuring the xanthan gum production levels of said selected rifampicin resistant colonies;
- and
- c) selecting those rifampicin resistant colonies which produce xanthan gum in excess to that produced by said parent strain.
Dependencies are inferred from the claim text.