US 5,310,676 AGranted

Cell cultivating device

AssigneeA/S Nunc

InventorsArne Johansson, Peter Esser

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Abstract

A cell cultivating device comprising superposed, separate partition wall members forming mutually spaced partition walls, which define superposed chambers for containing a cell cultivating medium therebetween. The superposed partition wall members each comprising said partition wall and an upstanding peripheral wall extending transversely thereto, are arranged within a closed outer container having a flat bottom wall and a flat end wall. A liquid cultivating medium may be introduced into the outer container through a filling opening while the container is in an upright position in which it is supported by the end wall. The liquid may then substantially uniformly distribute itself within the cultivating chambers via a tunnel extending along the end wall and interconnecting the cultivating chambers. The partition wall members are preferably sealingly interconnected along the periphery thereof, and the free end of the upper partition wall member may be spaced from the inner surface of the container top wall in order to reduce problem of leakage of liquid medium between the chambers.

Claims36

Dependent claims

A cell cultivating device comprising

an outer closable container having a substantially flat container bottom wall, a top wall, a substantially flat first end wall extending at substantially right angles to the flat container bottom wall, a second end wall, and opposite side walls interconnecting the top and container bottom walls and the end walls, one of said walls defining a closable filling opening therein,
an insert positioned within the outer container and including at least first and second interconnected partition wall members, each of the partition wall members including a flat chamber bottom wall extending substantially parallel with the flat container bottom wall and a peripheral wall surrounding the partition wall at its outer edge and extending therefrom toward the container top wall, each of said peripheral walls engaging with adjacent inner surface parts of the container walls, and said chamber bottom walls dividing an inner space defined by the outer container into superposed cultivating chambers,
passage means for mutually fluidly interconnecting all of the superposed cultivating chambers so that liquid introduced into the container via the closable filling opening may distribute itself in all of the interconnected chambers when the container is placed in an upright position in which it is supported by the flat first end wall, said passage means comprises an interconnecting passage defined adjacent to an inner side surface part of the flat first end wall, each chamber communicating with the interconnecting passage through a connecting opening provided in the respective peripheral wall extending along only a fraction of the width of the first end wall being spaced from the bottom wall of the respective cultivating chamber.

A cell cultivating device comprising an outer container, having a substantially flat container bottom wall, a container top wall, a substantially flat first end wall extending at substantially right angles to the flat container bottom wall, a second end wall, and opposite side walls interconnecting the container top and container bottom walls and the end walls, one of said walls defining a closable filling opening therein,

at least two superposed partition wall members dividing an inner space of the outer container into superposed cultivating chambers, each partition wall member having a flat chamber bottom wall and a surrounding peripheral wall extending from the chamber bottom wall towards the top container wall and engaging with adjacent inner surface parts of the container walls, the chamber bottom walls, which are mutually spaced and spaced from the container top and container bottom walls, respectively, extending substantially parallel with the container bottom wall, and adjoining partition wall members having their peripheral walls sealingly interconnected along at least a major part of the peripheries of the partition wall members, the partition wall members being spaced apart along any non-interconnected parts by said peripheries, and
liquid passage means for mutually and fluidly interconnecting all of the cultivating chambers so that liquid introduced into the container via the closable filling opening may distribute itself in all of the interconnected chambers when the container is placed in an upright position in which it is supported by the flat first end wall.

Dependencies are inferred from the claim text.