Fowlpox virus non-essential regions
AssigneeBritish Technology Group Limited
InventorsMatthew M. Binns, Michael E. G. Boursnell, Joan I. A. Campbell, Fiona M. Tomley
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Abstract
The invention relates to DNA from fowlpox virus (FPV) providing a non-essential region for the insertion of foreign genes thereinto and thence the construction of a vector for homologous recombination with a wild type FPV, whereby the resulting recombinant FPV can be used for vaccination of animals, especially chickens. In this invention, the non-essential region consists substantially of a length of DNA from the long unique sequence of the terminal inverted repeat (TIR) of FPV or from the region at FPV which corresponds approximately to the HindIII D fragment genes D8 and D9 in vaccinia virus.
Claims10
A recombinant fowlpox virus (FPV) which contains within the long unique sequence of the terminal inverted repeat of the fowlpox virus genome, at least one foreign gene and at least one sequence for regulating the expression of the or each foreign gene.
A cloning vector containing a fowlpox non-essential region (NER) sequence being interrupted by at least one foreign gene and at least one sequence for regulating the expression of the foreign gene, said NER sequence being homologously recombinable with sequence within the long unique sequence (LUS) of the terminal inverted repeat (TIR) of the fowlpox virus genome.
A DNA molecule which consists essentially of a fowlpox non-essential region (NER) sequence being interrupted by at least one foreign gene and at least one sequence for regulating the expression of the foreign gene, said NER sequence being homologously recombinable with sequence within the long unique sequence (LUS) of the terminal inverted repeat (TIR) of the fowlpox virus genome.
A recombination vector comprising a cloning vector, said cloning vector containing a fowlpox non-essential region (NER) sequence being interrupted by at least one foreign gene and at least one sequence for regulating the expression of a said foreign gene, said NER sequence being homologously recombinable with sequence within the long unique sequence (LUS) of the terminal inverted repeat (TIR) of the fowlpox virus genome.
Dependencies are inferred from the claim text.