Back to results
US 5,310,670 AGranted
Method for the purification of Erwinia L-asparaginase
AssigneePublic Health Laboratory Service Board
InventorChristopher R. Goward
Life of this patent
Estimating the term…
Abstract
A process is provided for the purification of L-asparaginase by contacting a crude extract of L-asparaginase with a solid medium having cation exchange groups so as to adsorb L-asparaginase on the support and eluting adsorbed L-asparaginase from the support. The cation exchange groups comprise sulphonate groups and the elution step is carried out at a pH which is higher than the pH used in the contacting step.
Claims10
A process for producing purified asparaginase which comprises the steps of
- (i) extracting L-asparaginase from disrupted cells at a pH of from 11.3 to 11.5,
- (ii) adjusting the pH to within the range 6.3 to 6.7,
- (iii) separating supernatant from precipitated impurities,
- (iv) subjecting supernatant from step (iii) to adsorption on a solid medium, having cation exchange groups so as to adsorb L-asparaginase on the solid medium wherein the cation exchange groups comprise sulphonate groups, and
- (v) eluting adsorbed L-asparaginase,
- and wherein the contacting of the supernatant with the solid medium in step (iv) is effected at a pH of from 4.0 to 5.5, and wherein the eluting of adsorbed L-asparaginase in step (v) is effected at a pH of from 6.0 to 7.5.
3 more elements
Dependencies are inferred from the claim text.