Site specific double stranded DNA endonuclease
AssigneeBoard of Regents, The University of Texas System
InventorsRonald A. Butow, Philip S. Perlman
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Abstract
An endonuclease which cleaves double stranded DNA at a specific site, producing staggered ends with 4 base pair, 3' overhangs, has been isolated, purified and characterized. A method of producing sufficient quantities of the endonuclease for purification and characterization is disclosed. A method to use the endonuclease to cleave DNA, producing fragments useful for gene mapping is also disclosed.
Claims16
An endonuclease protein encoded in the fourth intron of yeast cytochrome oxidase having an apparent molecular weight of about 29,000 daltons as determined by SDS-PAGE, and which is capable of cleaving double-stranded DNA at a site identified as follows:
- ##STR2##
Dependencies are inferred from the claim text.