Affinity peptides
AssigneeHoffman-La Roche Inc.
InventorsHeinz Dobeli, Bernhard Eggimann, Reiner Gentz, Erich Hochuli, Dietrich Stuber
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Abstract
Fusion proteins and a process for their purification by means of metal chelate affinity chromatography on NTA resins are provided by this invention.
Claims11
A DNA sequence coding for a fusion protein comprising a biologically active polypeptide or protein linked by its amino- or carboxy-terminus to one or two affinity peptides, which peptides have from 2 through 6 adjacent histidine residues:
A recombinant vector comprising a vector and a DNA sequence coding for a fusion protein comprising a biologically active polypeptide or protein linked by its amino- or carboxyl-terminus to one or two affinity peptides, which peptides have from two through six adjacent histidine residues, wherein the recombinant vector is capable of directing expression of the DNA sequence in a compatible unicellular host organism.
A unicellular organism containing a recombinant vector comprising a vector and a DNA sequence coding for a fusion protein comprising a biologically active polypeptide or protein linked by its amino- or carboxyl-terminus to one or two affinity peptides, which peptides have from two through six adjacent histidine residues, wherein the unicellular organism is capable of expressing the DNA sequence.
A process for producing a fusion protein comprising:
- (a) culturing a unicellular organism containing a recombinant vector comprising a vector and a DNA sequence coding for a fusion protein comprising a biologically active polypeptide or protein linked by its amino- or carboxyl-terminus to one or two affinity peptides, which peptides have from two through six adjacent histidine residues, wherein the unicellular organism is capable of expressing the DNA sequence, under conditions in which the fusion protein is produced;
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- (b) isolating the fusion protein from the culture.
Dependencies are inferred from the claim text.