Nucleic acid encoding the .alpha. chain prodomains of inhibin and method for synthesizing polypeptides using such nucleic acid
AssigneeGenentech, Inc.
InventorsAnthony J. Mason, Peter H. Seeburg
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Abstract
DNA encoding the prepro inhibin .alpha. and .beta. chains has been isolated. This DNA is ligated into expression vectors and used to transform host cells for the preparation of inhibin or activin. Also provided are prohormone domains and other inhibin .alpha. or .beta. chain derivatives having therapeutic or diagnostic interest. The compositions provided herein are useful in the manipulation of fertility in animals.
Claims16
A method for producing an inhibin .alpha. chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-230 of FIG. 1B, and amino acid residues 1-29 and 30-199 of FIG. 6, comprising (a) constructing a vector that comprises a nucleic acid encoding said prodomain, (b) transforming a host cell with the vector, and (c) culturing the transformed cell under conditions appropriate for expression of said polypeptide.
Non-chromosomal DNA encoding an inhibin .alpha. chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-230 of FIG. 1B, and amino acid residues 1-29 and 30-199 of FIG. 6.
A replicable vector comprising DNA encoding an inhibin .alpha. chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-230 of FIG. 1B, and amino acid residues 1-29 and 30-199 of FIG. 6.
A host cell transformed with a replicable vector comprising DNA encoding an inhibin .alpha. chain prodomain polypeptide comprising a sequence selected from the group consisting of amino acid residues 1-230 of FIG. 1B, and amino acid residues 1-29 and 30-199 of FIG. 6.
Dependencies are inferred from the claim text.